Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Locali
As mucus is highly hydrated, special care has to be taken to preserve this in hi
As mucus is highly hydrated, special care has to be taken to preserve this in histological preparations during immunostaining. Here, we describe how to fix tissues in such a way that the mucus is preserved in paraffin-embedded tissue. We also describe how the major macromolecular components in the mucus, the mucins, are immunostained and how bacteria can be localized in preparations with preserved mucus by fluorescent in situ hybridization.
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Cloning Gene Family Members Using Polymerase Chain Reaction with Degenerate Oligonucleotide Primers
As more and more genes are cloned and sequenced, it is appar...
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Small-Angle Neutron Scattering for Molecular Biology: Basics and Instrumentation
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