Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry

In this chapter, a concise account of the synthesis of oligonucleotides using the H -phosphonate methodology is given. It includes various methods for the preparation of the starting material, nucleoside 3′-H -phosphonate monoesters, their conversion into dinucleoside H -phosphonate diesters, oxidative transformations of dinucleoside H -phosphonates into the corresponding phosphate and phosphorothioate derivatives, and protocols for the synthesis of oligonucleotides and their phosphorothio analogs.