Radiolabeling of DNA Using Polynucleotide Kinase
Labeling of DNA fragments at the 5′ end with polynucleotide kinase is one of the
Labeling of DNA fragments at the 5′ end with polynucleotide kinase is one of the methods currently employed to obtain highly labeled DNA for sequencing purposes. This end-labeled DNA is used in the partial chemical degradation method for sequencing DNA (see Chapter 51 and ref. 1 ).
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