Detection of anti-tumor immunity induced by laser immunotherapy

Metastatic mammary tumors in rats were treated by laser immunotherapy. Tumors injected by a laser-absorbing dye and an immunoadjuvant were irradiated non-invasively by a near-infrared laser. The successfully cured rats developed a long-term resistance to repeated tumor challenges. Using the sera from cured rats as the source of primary antibodies, immune responses induced by laser immunotherapy were observed in both cellular and molecular levels using histochemical assays and Western blot analysis.