Bioluminescence-Based Metal Detectors

This chapter consists of two methodologically rather divergent topics: the construction of a metal sensor bacterial strain and the measurement of bioavailable metal using such a strain. The constructton part IS written assummg that readers are famthar with basic recombinant DNA techniques, such as isolation and purificatron of DNA, the use of restriction enzymes, and ligation. However, if that is not the case, plasmids and bacterial strains for metal bioavailabtlity measurements are avarlable from the authors’ laboratory.