常用的β-actin 引物序列

human actin f ctc cat cct ggc ctc gct gt

human actin r gct gtc acc ttc acc gtt cc

product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit actin f tgg ctc taa cag tcc gcc ta

product size:295 mouse actin r cgt tga cat ccg taa aga cc mouse actin f aac agt ccg cct aga agc ac

product size:281 rat actin f TCAGGTCATCACTATCGGCAAT rat actin r AAAGAAAGGGTGTAAAACGCA

product size:432 human actin r gag cta cga gct gcc tga cg human actin f cct aga agc att tgc ggt gg

product size:416 mouse actin f tca tca cta ttg gca acg agc mouse actin r aac agt ccg cct aga agc ac

product size:399 rat actin f CCCATCTATGAGGGTTACGC rat actin r TTTAATGTCACGCACGATTTC

product size:150 rabbit actin f tct tcc agc cct cct tcc tg rabbit actin r cgt ttc tgc gcc gtt agg t

product size:409

内参基因名称 引物 引物最佳退火 扩增

基因库序列号 引物名称 序列 位置 Tm 温度C 长度 Human actin beta F305 ctgggacgacatggagaaaa 305-324 52.3

BC002409 R868 aaggaaggctggaagagtgc 868-849 52.6 59.4 564

F1379 agcgagcatcccccaaagtt 1379-1398 57.3

R1663 gggcacgaaggctcatcatt 1663-1644 56.3 54 285 Rat actin beta F18 cacccgcgagtacaaccttc 18-37 54.5

NM_031144 R224 cccatacccaccatcacacc 224-205 54.4 60.4 207

F694 gagagggaaatcgtgcgtgac 694-714 54

R1146 catctgctggaaggtggaca 1146-1127 53.2 57.1 452 Mouse actin beta F91 atatcgctgcgctggtcgtc 91-110 57.5

NM_007393 R607 aggatggcgtgagggagagc 607-588 57.8 60.4 517

F1566 gtccctcaccctcccaaaag 1566-1585 54.

F1831 gctgcctcaacacctcaaccc 1831-1811 54.4 55.7 266 human GAPDH F369 agaaggctggggctcatttg 369-388 55.6

BC004109 R626 aggggccatccacagtcttc 626-607 55.1 57.5 258

F66 aggtcggagtcaacggatttg 66-86 52.5

R597 gtgatggcatggactgtggt 597-578 52.6 57 532 Rat GAPDH F50 cagtgccagcctcgtctcat 50-69 55

BC059110 R644 aggggccatccacagtcttc 644-625 55.1 58.3 595

F1029 acagcaacagggtggtggac 1029-1048 54.4

R1280 tttgagggtgcagcgaactt 1280-1261 54.8 57.7 252 Mouse GAPDH F306 aggccggtgctgagtatgtc 306-325 54

M32599 R835 tgcctgcttcaccaccttct 835-816 54.6 58.7 530

F49 ggtgaaggtcggtgtgaacg 49-68 55.1

R281 ctcgctcctggaagatggtg 281-262 54.5 56.8 233 Rat 18S rRNA F1865 tgcggaaggatcattaacgga 1865-1886 58.6

M11188 R2164 agtaggagaggagcgagcgacc 2164-2143 59 59.3 300 human 18S rRNA F1565 cagccacccgagattgagca 1565-1584 58.3

M10098 R1816 tagtagcgacgggcggtgtg 1816-1797 58.3 59 253 mouse 18S rRNA F56 aggggagagcgggtaagaga 56-75 54.4

K013364 R296 ggacaggactaggcggaaca 296-277 54 62 241