PCR clean up using 3M sodium acetate and chilled absolute ethanol

For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube.

• 2 ul of 3M sodium acetate (NaOAc) pH 4.5
• 40 ul chilled absolute ethanol.

Transfer the PCR product (20ul) into the tube containing the mixture. Vortex the tube and then store it at -20 for 30-50 min. Spin for 30 min at maximum speed 14.000 rpm. Rremove the supernatant carefully and don''t disturb the pellet. ( Do not touch the bottom of the tube) Wash the pellet with 70% ethanol. And spin the tube at maximum speed for 5 min. Remove the supernatant and then dry the pellet using vacuum centrifuge. (do not apply heat) Resuspend the pellet in 30 ul in sterile water.