Western Blot with BSA

Blotting with BSA

1. Blot in 5% mile/ 1X PBS T (or 1X TBST) --60'/Rt

2. Wash 2X for 5' in 1XPBST

3. 10Ab in 1% BSA/1X PBST --60'/Rt

4. Wash PBST 15', then 3x for 5' each in 1X PBST

5. 20Ab in 1% BSA/1X PBST --60'/Rt (aHRP)--

6. Wash 15' with 1X PBST then 3x with PBST for 5' 

7. Chemiluminescence: Mix 1:1 NEN reagents. Add to Mb --1'/shaking. Blot excess

8. Put between sheets (page protector) and expose film