Gel Mobility Shift Analysis

Material/reagents:

• 2X binding buffer:       20 mM Tris pH 7.5       100 mM NaCl       2 mM EDTA       10% glycerol

• poly(dI-dC) (5 mg/ml in TE)

• BSA (10 mg/ml)

• 32P-labeled probe

• Buffer C:       20 mM HEPES pH 7.9       25% glycerol       420 mM NaCl       1.5 mM MgCl2

• 4% native polyacrylamide gel (50 ml):       5 ml 40% acrylamide (30:1)       2.5 ml 5X TBE       41.95 ml H20       0.5 ml 10% APS       50 ml TEMED

• 0.25X TBE running buffer

• Loading dye:       0.2% bromophenol blue       0.2% xylene cyanol       50% glycerol       0.5 mM DTT       0.5 mM PMSF       0.2 mM EDTA

具体操作流程请点击下面的链接下载：

Gel Mobility Shift Analysis .pdf