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Western Blot化学发光(ECL)淬灭的原因？
做Western Blot发光实验经常有“淬灭”的情况发生，经常有朋友反映，加发光试剂后立刻可以看到很明显的亮光，可亮光很快就消逝了，压完片后，条带却很弱，甚至
2025-01-07 -
PCR 标记DNA
PCR 是通过热稳定酶，在模板、dNTPs 和一组与片段3′ 序列互补的引物存在下，对插入物进行次序扩增(Saiki et al. 1988) 。PCR 技术提
2025-01-07 -
western-blot化学发光时，出现非特异条带，该怎么办？
出现杂带或非特异条带的原因可能有以下几个方面：1. 蛋白上样量过大。可降低蛋白上样量。2. 一抗是多克隆抗体，或一抗、二抗浓度太高。可更换成单克隆抗体，或降低抗
2025-01-07 -
PCR实验最常见的9个问题及解决方案
1. 如何有效设计引物（1）使用Oligo或Primer设计引物，在保守区内设计，长度一般在18-27bp，上下游引物Tm值最好是60-75℃，GC含量=40%
2025-01-07 -
限制酶和DNA 甲基化酶
限制酶特异性地结合于一段被称为限制酶识别序列的特殊DNA 序列之内或其附近的特异位点上，井在此切割双链DNA 。它可分3 类，分别是第一型（Type I）、第二
2025-01-07 -
SDS 碱裂解法制备质粒DNA: 大量制备
近些年来，随着PCR 技术的出现，以及DNA 克隆和测序方法的发展，对大量质粒载体和重组子的需求也大大减少。因此，本方案曾一度广泛应用，但已经被更为快速和简便的
2025-01-07 -
RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium
Knockouts by homologous recombination are frequently used to investigate the fun
2025-01-06 -
合子基因（zygotic gene）
受精卵在胚胎发育过程中表达的基因称为合子基因，受母体基因产物的激活。合子基因的表达是胚胎模式形成的开端。合子基因分为若干组，表达顺序有先有后，它们之间有着严密的
2025-01-06 -
Phylogenetic Inference and Parsimony Analysis
Application of phylogenetic inference methods to comparative endocrinology studi
2025-01-06 -
Knock-Down of Gene Expression in Hematopoietic Cells
RNA interference (RNAi) is an evolutionarily conserved sequence-specific post-tr
2025-01-06 -
Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protei
The insulin-like growth factor-binding proteins (IGFBPs) are carrier proteins th
2025-01-06 -
Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products
Cell-cell electrofusion (CCE) is a process that involves forcing cells into clos
2025-01-06 -
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS :1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2
2025-01-06 -
Long-Term Cryopreservation of Thylakoid Membranes
Chloroplast thylakoids are the site of photosynthetic light reactions, electron
2025-01-06 -
Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells
Since the mid-1980s, murine retroviral vectors have been used extensively by a n
2025-01-06
