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PCR扩增产物出现杂带怎么办?
可能的原因和对应的解决方案如下:1.引物用量偏大,引物的特异性不高。应调换引物或降低引物的使用量。2.循环的次数过多。适当增加模板的量,减少循环次数。3.酶的用
2025-01-06 -
Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy
The microtubule-targeting agents have made significant contributions to cancer t
2025-01-06 -
Whole mount TUNEL analysis of Xenopus embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8)
2025-01-06 -
Western blot实验最常见的10个问题及解决方案
1、Western Blot原理Westernblot的原理是蛋白质在电力场的作用下,由大到小的进行排列,利用电泳进行分离和富集。拥有抗原表位的蛋白质分子被抗体
2025-01-06 -
Class II MHC Antigen Processing in Phagosomes
Phagocytic antigen-presenting cells (APCs) are involved in innate and adaptive i
2025-01-06 -
分子伴侣(molecular chaperones)
分子伴侣是细胞中一大类蛋白质, 是由不相关的蛋白质组成的一个家系,它们介导其它蛋白质的正确装配,但自己不成为最后功能结构中的组分。分子伴侣的概念有三个特点:①凡
2025-01-06 -
慢病毒感染细胞后,细胞长满48小时是否可以先传代,再等到72小时再喷霉素筛选吗
通常在进行慢病毒感染后,建议遵循以下操作步骤:感染后观察细胞状态:感染后一般在48-72小时内可以看到较为明显的表达,但是否开始筛选需要看细胞的状态和感染效率。
2025-01-06 -
Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells: Genetic Selection and Potentia
Embryonic stem (ES) cells are pluripotent cells derived from blastocyst-stage em
2025-01-06 -
Synchronization of Yeast
The budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces
2025-01-06 -
Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to
Electrophoretic mobility shift assay and Western blot are simple, efficient, and
2025-01-06 -
小鼠白细胞介素22(IL-22)酶联免疫分析
小鼠白细胞介素22(IL-22) 酶联免疫 分析试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中白细胞介素22(IL-
2025-01-06 -
Western blot 中Tween-20的作用是什么?应该怎么用?
Western blot的洗液TBST是实验室常用的配制液体,对于TBST的配方也基本上相残不多,其中配方中就有一种组分是Tween-20,一种黄色或琥珀色澄明
2025-01-06 -
Cell Lineage Analysis in Xenopus Embryos
Cell lineage studies reveal what kinds of tissues descend from a single cell or
2025-01-06 -
Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration
The function of many glycoproteins is directly related to the structure of their
2025-01-06 -
蛋白质的保存方法如何选择?
保存方法的选择取决于几个因素,包括蛋白质内在的稳定性、所需的保存时间的长短和对于防止破坏所要求的程度。这些因素有很大的变异性,但是,在大多数情况下都应当遵守以下
2025-01-06