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  • PCR扩增产物出现杂带怎么办?

    PCR扩增产物出现杂带怎么办?

    可能的原因和对应的解决方案如下:1.引物用量偏大,引物的特异性不高。应调换引物或降低引物的使用量。2.循环的次数过多。适当增加模板的量,减少循环次数。3.酶的用

    2025-01-06
  • Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy

    Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy

    The microtubule-targeting agents have made significant contributions to cancer t

    2025-01-06
  • Whole mount TUNEL analysis of Xenopus embryos

    Whole mount TUNEL analysis of Xenopus embryos

    Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8)

    2025-01-06
  • Western blot实验最常见的10个问题及解决方案

    Western blot实验最常见的10个问题及解决方案

    1、Western Blot原理Westernblot的原理是蛋白质在电力场的作用下,由大到小的进行排列,利用电泳进行分离和富集。拥有抗原表位的蛋白质分子被抗体

    2025-01-06
  • Class II MHC Antigen Processing in Phagosomes

    Class II MHC Antigen Processing in Phagosomes

    Phagocytic antigen-presenting cells (APCs) are involved in innate and adaptive i

    2025-01-06
  • 分子伴侣(molecular chaperones)

    分子伴侣(molecular chaperones)

    分子伴侣是细胞中一大类蛋白质, 是由不相关的蛋白质组成的一个家系,它们介导其它蛋白质的正确装配,但自己不成为最后功能结构中的组分。分子伴侣的概念有三个特点:①凡

    2025-01-06
  • 慢病毒感染细胞后,细胞长满48小时是否可以先传代,再等到72小时再喷霉素筛选吗

    慢病毒感染细胞后,细胞长满48小时是否可以先传代,再等到72小时再喷霉素筛选吗

    通常在进行慢病毒感染后,建议遵循以下操作步骤:感染后观察细胞状态:感染后一般在48-72小时内可以看到较为明显的表达,但是否开始筛选需要看细胞的状态和感染效率。

    2025-01-06
  • Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells: Genetic Selection and Potentia

    Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells: Genetic Selection and Potentia

    Embryonic stem (ES) cells are pluripotent cells derived from blastocyst-stage em

    2025-01-06
  • Synchronization of Yeast

    Synchronization of Yeast

    The budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces

    2025-01-06
  • Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to

    Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to

    Electrophoretic mobility shift assay and Western blot are simple, efficient, and

    2025-01-06
  • 小鼠白细胞介素22(IL-22)酶联免疫分析

    小鼠白细胞介素22(IL-22)酶联免疫分析

    小鼠白细胞介素22(IL-22) 酶联免疫 分析试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中白细胞介素22(IL-

    2025-01-06
  • Western blot 中Tween-20的作用是什么?应该怎么用?

    Western blot 中Tween-20的作用是什么?应该怎么用?

    Western blot的洗液TBST是实验室常用的配制液体,对于TBST的配方也基本上相残不多,其中配方中就有一种组分是Tween-20,一种黄色或琥珀色澄明

    2025-01-06
  • Cell Lineage Analysis in Xenopus Embryos

    Cell Lineage Analysis in Xenopus Embryos

    Cell lineage studies reveal what kinds of tissues descend from a single cell or

    2025-01-06
  • Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration

    Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration

    The function of many glycoproteins is directly related to the structure of their

    2025-01-06
  • 蛋白质的保存方法如何选择?

    蛋白质的保存方法如何选择?

    保存方法的选择取决于几个因素,包括蛋白质内在的稳定性、所需的保存时间的长短和对于防止破坏所要求的程度。这些因素有很大的变异性,但是,在大多数情况下都应当遵守以下

    2025-01-06