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Using the Fluorescent Styryl Dye FM1-43 to Visualize Synaptic Vesicles Exocytosis and Endocytosis in
The styryl dye FM1-43 is a powerful tool to track exocytosis, endocytosis and re
2025-02-28 0 -
Liposome Formulations of Hydrophobic Drugs
Here, we report methods of preparation for liposome formulations containing lipo
2025-02-28 1 -
Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe
Eukaryotic cells have the ability to influence progression through the cell cycl
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In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination
Posttranslational histone modifications play important roles in regulating chrom
2025-02-28 1 -
小鼠胚胎干细胞培养实验步骤
相关专题以下是根据NIH老年病研究所提供的英文的R1胚胎干细胞培养 protocol整理而成。根据经验作部分修改。1、一般培养-保持胚胎干细胞 处于未分化状态培
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干细胞成骨诱导后的染色方法和步骤
碱性磷酸酶(ALP)是成熟成骨细胞的标志性酶,同时成骨细胞形成的钙结节也是成骨细胞的标记物。以ALP为目标物的检测方法:1 Gomori钙钴法【染色原理】ALP
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Measuring FRET Using Time-Resolved FLIM
Cell migration is a process that is controlled by the formation and correct loca
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Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes
Intravital microscopy (IVM) allows for the direct in vivo visualization of dynam
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Regulation of Krpple-Like Factor 5 by Targeted Protein Degradation
Transcription factors are usually unstable proteins. The degradation of the majo
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间隙连接(gap junction)
间隙连接是动物细胞中通过连接子(connexons)进行的细胞间连接。所谓“间隙”,有两层含义,其一是在间隙连接处, 相邻细胞质膜间有2~3nm的间隙;其二是在
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Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Growth factor stimulation results in phosphorylation of histone H3 at ser 10 and
2025-02-28 0 -
FACS Analysis Using Peripheral Blood Cells
FACS Analysis Using Peripheral Blood Cells Collect blood (75 microliters) into 1
2025-02-28 1 -
Staining Methods for cell death
Staining Methods for cell death Z. Xia 10/2/95 The simplest way: trypan blue. De
2025-02-28 1 -
A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar
This introductory article describes an episode that took place in the mid-1980s
2025-02-28 0 -
羊水细胞染色体标本制备
相关专题 一、原理 人的羊水细胞能长成单层并可连续进行传代培养,但它们的一些特征与一般成纤维细胞不同。在羊水中存在着各种不同类型的胎儿细胞,依据其外形和生长特
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