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Culturing HEK 293 Cells
ReagentsMedium: 500 ml Dulbecco’s Modified Eagle Medium (Gibco #41966-029) 55 ml
2025-02-12 1 -
微生物污染的排除
相关专题 细胞受各种霉菌、细菌 和支原体污染后，一般都较难排除或杀灭，其中以支原体更难排除，因此从预防着眼为上。 1） 抗生素除菌法：用BM－1（截耳素衍生
2025-02-12 3 -
The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV
The human papillomaviruses (HPVs) form a large group of small double-stranded DN
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Hematopoietic Colony-Forming Cells
The hematopoietic progenitors that can be assayed in clonal culture systems repr
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The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcrip
Developmental and homeostatic remodeling of the extracellular matrix (ECM) is a
2025-02-12 1 -
C4途径(C4 pathway)
有一些植物对CO2 的固定反应是在叶肉细胞的胞质溶胶中进行的,在磷酸烯醇式丙酮酸羧化酶的催化下将CO2 连接到磷酸烯醇式丙酮酸(PEP)上.形成四碳酸:草酰乙酸
2025-02-12 2 -
T cell Activation Protocol
Mature T cells recognize and respond to the antigen/MHC complex through their an
2025-02-12 1 -
Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos
In our efforts to use confocal laser scanning microscopy for study of organogene
2025-02-12 3 -
ELISPOT (Enzyme-linked ImmunoSPOT)
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally de
2025-02-12 1 -
三羧酸循环(citric acid cycle)
由乙酰CoA和草酰乙酸缩合成有三个羧基的柠檬酸, 柠檬酸经一系列反应, 一再氧化脱羧, 经α酮戊二酸、 琥珀酸, 再降解成草酰乙酸。而参与这一循环的丙酮酸的三个
2025-02-12 2 -
细胞培养中的黑胶虫污染问题的处理
相关专题我们实验室最近一次的情况:我们前后从上海细胞中心买了三批细胞，细胞刚到的时候，观察均生长良好，密度适中，培养液清亮，也没有见小黑点，但是对细胞传代以后就
2025-02-12 4 -
The Cell Environment (including types of culture medium)
In general terms cultured cells require a sterile environment and a supply of nu
2025-02-12 2 -
杂交瘤细胞的制备方法
1） 瘤细胞培养（无特殊要求）骨髓瘤细胞呈悬浮或轻微附着形式生长，如附着性生长的细胞多时，用吸管轻轻吹打或轻轻叩击培养容器即可分离下来。通常要维持细胞于指数生长
2025-02-12 3 -
红细胞裂解的实验方法步骤
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric
2025-02-12 3 -
Craniofacial Development of Avian and Rodent Embryos
In this overview, we examine approaches to the embryological development of the
2025-02-12 3
