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细胞增殖检测方法汇总
细胞增殖检测法(一):直接计数法这是一种非常简单的检测方法,我们可以利用计数板或技术仪得出细胞数目然后对数目进行比较即可。其缺点是不适合评估特定亚群、和数量较多
2025-02-11 0 -
Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Nativ
Voltage-gated K+ channels (KV channels) are encoded by the KCNx gene family and
2025-02-11 1 -
Isolation and Characterization of Cancer Stem Cells In Vitro
The cancer stem cell hypothesis is an appealing concept to account for intratumo
2025-02-11 1 -
Cell-Based Co-transfection Microarrays for Use with HEK293T Cells on a Poly d-Lysine-Coated Polystyr
Analysis of the human genome sequence has identified thousands of putative genes
2025-02-11 0 -
Mosaic Analysis in Caenorhabditis elegans
The complete description of its nearly invariant cell lineage (1 ,2 ) and the gr
2025-02-11 1 -
Fine Structure of the Autophagosome
This chapter describes the electron microscopic fine structure of early and late
2025-02-11 0 -
Antisense Therapeutics in the Central Nervous System: The Induction of c-fos
Immediate-early genes (IEGs) are members of a class of genes that respond, in ma
2025-02-11 0 -
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates
We present a method that uses microcontact printing of alkanethiols on gold to g
2025-02-11 0 -
Determination of Gene Promoter Activity in Skeletal Muscles In Vivo
The use of nonviral (plasmid DNA) gene delivery into skeletal muscle has increas
2025-02-11 0 -
Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics
Microscopic imaging of single fluorescent molecules within cells provides a mole
2025-02-11 0 -
Application of Differential Display in Studying Developmental Processes
Developmental processes are intricate networks of events by which a fertilized e
2025-02-11 0 -
Preparation of RNA from Cultured Cells
Preparation of RNA from Cultured CellsNotes: When preparing solutions for use wi
2025-02-11 1 -
Fertilization and Sperm Chemotaxis in Ascidians
Prior to fertilization, spermatozoa of many animals and plants show chemotactic
2025-02-11 0 -
Derivation of High-Purity Oligodendroglial Progenitors
Oligodendrocytes are a type of glial cells that play a critical role in supporti
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A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA
Oligonucleotide-directed site-specific mutagenesis is a powerful tool to explore
2025-02-11 2