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成熟针叶林针叶和针叶树韧皮组织总RNA提取
Many different methods have been tried in an effort to extract quality RNA from
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Isolation of total RNA using CsCl/EtBr gradients--密度梯度法提取总RNA
RNA for S1 or PE analysis must be free of chromosomal DNA. This can be accomplis
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TRIzol method for RNA isolation
TRIzol method for RNA isolation-To tissue or cells, add 200- 1 ml TRIzol (GIBCO
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提取棉花RNA--RNA EXTRACTION METHOD FOR COTTON
Based on: Wan, C.-Y. and Wilkins, T.A. 1994. Anal. Bioch. 223:7-12.1.Collect you
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从土壤中轻松获取高质量RNA
众所周知，从土壤中提取DNA已非易事，而想从中再提取RNA可说是难上加难。DNA不容易提取是因为本身土壤中富集的材料就少，提取过程相对复杂，造成操作损失；而RN
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How to isolate mRNA(分离mRNA)
Background: mRNAs （messenger RNAs） comprise only a small percentage of all RNA s
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CONCERT细胞质RNA纯化试剂
CONCERT细胞质RNA 纯化试剂是一种新颖的单相酚溶液，是专门设计用来从新鲜或冷冻的培养的动物细胞 或组织中快速、简单地纯化高质量的细胞质RNA 。应用利用
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变性胶体电泳与北方杂合分析
mRNA为单股，一般会卷绕成特定的三级结构，并与某些蛋白质结合，而以messenger ribonucleoprotein particles （mRNPs）
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Preparation of total RNA from whole blood collected in PAXgene tubes
Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; o
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如何选择细胞平衡盐缓冲液D-PBS、EBSS、HBSS
细胞学实验中必不可少的试剂就是平衡盐溶液，它可以保护细胞免受pH波动的影响，平衡细胞膜的渗透压。平衡盐溶液通常包括钠 (Na)、磷酸钠 (Na3
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Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
Prior to Extractions ・ Bake all necessary glassware, metal spatulas, mortars, an
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DNAse Treatment Of Total RNA
OverviewMost RNA extractions procedures yield RNA, with minimal or no contaminat
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哺乳动物细胞总RNA的分离
这一从培养的单层哺乳动物细胞中分离RNA的实验程序系为Favaloro 等（1980）所介绍程序的改良。该程序同样适用于从悬浮培养的哺乳动物细胞中或从易于分散成
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Preparation of total cellular RNA--提取总RNA方法
1) Harvest 1x105 to 108 cells. Wash 1x w/PBS and freeze in liquid N2 and store @
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植物组织mRNA提取
由于mRNA末端含有多poly（A）+,当总RNA流径oligo（dT）纤维素时，在高盐缓冲液作用下，mRNA被特异的吸附在oligo（dT）纤维素柱上，在低盐
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