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Thawing Cells (Schreiber protocol)
Thawing Cells (Schreiber's protocol) Thaw vial quickly in 37C water. Caution
2025-03-06 2 -
细胞培养室的建立
前段时间，经历了几个月的时间，为实验室建了一个细胞培养间。目前这个细胞培养间运转良好，培养的神经元长了 10 来天，长势不错，已经可以认为细胞培养室建立成功。在
2025-03-06 1 -
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
Aim DNA staining methods such as Hoechst staining techniques are quick with resu
2025-03-06 3 -
DNA疫苗实验方法
For direct gene transfer of tibialis anterior (TA) muscle in mice: It is optima
2025-03-06 0 -
自由扩散
物质通过质膜进出细胞的方式之一。物质从高浓度一侧通过质膜向低浓度一侧的扩散，不需要载体也不消耗能量。物质的自由扩散速度，与物质的脂溶性程度、膜两侧溶质浓度差、溶
2025-03-06 0 -
Tips for Tissue Culture
Cells attach to the culture substratum with varying degrees of adherence (see Ch
2025-03-06 0 -
Akt信号通路总况
相关专题 Akt1, also known as "Akt" or protein kinase B (PKB). Its signalli
2025-03-06 0 -
Classical and Molecular Cytogenetic Analysis
Cytogenetic analysis is performed on cell cultures for several reasons, notably,
2025-03-06 0 -
外周血培养基配制
一、配制用水 培养基的大部分是水，所以水的质量直接影响培养基的质量。按Waymouth标准，水的电阻应为200万欧姆，一般实验室里以玻璃蒸馏器制备的 双蒸水或三
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Isoelectric Focusing (IEF) of Proteins in Thin-Layer Polyacrylamide Gels
Isoelectric focusing (IEF) is an electrophoretic method for the separation of pr
2025-03-06 0 -
真核细胞蛋白表达疑难求解!
从Yeast Two-Hybrid中筛出一个蛋白,体外实验很成功,但在293中表达不成功.已经尝试了多种载体,包括pCMV-tag 3B,pcDNA3,pcDN
2025-03-06 1 -
Master Cell Bank
PurposeTo describe the preparation of a Master Cell BankSafetySee SP 09-001 for
2025-03-06 1 -
4小时51Cr释放法检测CMC活性
一、用 51 Cr 铬酸钠标记靶细胞 短期标记法 1 ．用 RPMI-1640 培养液洗涤 107 靶细胞，去上清液。用 0.5ml 不含碳酸氢钠的完全培养液悬
2025-03-06 2 -
The Generation of Multicopy Recombinant Strains
The extremely high levels of alcohol oxidase produced from the native AOX1 gene
2025-03-06 1 -
真细菌(Bacteria, eubacteria)
除古细菌以外的所有细菌均称为真细菌。最初用于表示“真”细菌的名词主要是为了与其他细菌相区别。
2025-03-06 3
