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II型内含子(group II introns)
主要存在于线粒体中的一类内含子，它的剪接位点类似于核编码结构基因的内含子,并同样遵从GT--AG规律。剪接机理同核内含子的剪接相似,也要形成一个套索的中间体,通
2025-03-06 3 -
Dorsal Root Ganglion Neuron Culture from Adult Rats
Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) n
2025-03-06 3 -
孚尔根[Feulgen]核反应染色法
相关专题 一、实验目的 学习和掌握孚尔根反应染色法，鉴定植物细胞核 内染色体上DNA的存在。 二、实验原理 DNA是主要的遗传物质，集中于染色体 上。1924
2025-03-06 5 -
细胞生长检测3：XTT检测法
XTT 检测法 用 XTT 代替 MTT 可省去溶解还原产物结晶的步骤， XTT 可以被活细胞中的代谢酶还原成黄色水溶性的代谢产物（ formazan ）。代谢
2025-03-06 3 -
Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach
Oxidative stress has been shown to be one of the mechanisms involved in a number
2025-03-06 2 -
Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy
RNA interference or RNAi-based gene therapy for the treatment of HIV-1 infection
2025-03-06 2 -
小鼠胚胎成纤维细胞MEF培养相关知识总结
小鼠胚胎成纤维细胞的富集1、给13-14天的孕鼠注射大约0.5ml阿佛丁。当鼠麻醉后，实施断颈法处死小鼠。2、用70％乙醇擦拭腹部，把皮肤向后拉，暴露出腹膜。用
2025-03-06 2 -
紧密连接
紧密连接有两种英文名称,同一个意思(tight junction ,zonula occludens),又称为不通透连接(impermeable junctio
2025-03-06 2 -
Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluore
2025-03-06 1 -
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
Description This protocol is for use with the D cyclins and employs 488 nm argon
2025-03-06 1 -
Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections
Immunohistochemistry is a powerful technique to localize proteins in tissues and
2025-03-06 1 -
Online Tools for Predicting Integral Membrane Proteins
We identify and describe a set of tools readily available for integral membrane
2025-03-06 0 -
核酸酶保护分析简介
相关专题 核糖核酸酶保护实验核酸酶保护法是一种用于生物化学和遗传学识别个人在异构RNA的提取样品RNA分子细胞实验室技术。 该技术可以识别一个或多个已知序列的R
2025-03-06 0 -
Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D
Phospholipase D (PLD) hydrolyzes phosphatidylcholine to produce the membraneasso
2025-03-06 0 -
转基因――材料和试剂
Media and Solutions Required for Routine ES Cell Culture: Version 1 (PMCI) Medi
2025-03-06 0
