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Relative Reverse Transcription-Polymerase Chain Reaction
A number of methods are available for assessing messenger ribonucleic acid (mRNA
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Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
The suppressors of cytokine signalling (SOCS) protein family consist of eight me
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Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media
Generic methods for genetic manipulation of human embryonic stem cells (hESCs) a
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膜结合蛋白(membrane-binding proteins)
是非肌细胞质膜下方产生收缩的机器。在剧烈活动时，由收缩蛋白作用于质膜产生的力引起质膜向内或向外移动(如吞噬作用和胞质分裂)。这种运动由肌动蛋白纤维直接或间接与质
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Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling
Use of loss-of function (via antisense Morpholino oligonucleotides (MOs)) or ove
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Isolation and Purification of Murine Male Germ Cells
Seminiferous tubules of the male testis contain somatic cells (Sertoli and Leydi
2025-02-12 2 -
Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential
Here we describe the preparation of nuclear extracts and the electrophoretic mob
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ATP-Loaded Liposomes for Targeted Treatment in Models of Myocardial Ischemia
ATP cannot be effectively delivered to most tissues including the ischemic myoca
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Designing RNAi Screens to Identify JAK/STAT Pathway Components
The JAK/STAT signaling pathway has essential roles in multiple developmental pro
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Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
Fluorescence correlation spectroscopy (FCS) is an emerging technique employed in
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Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology
The recently developed near-infrared (NIR) light imaging technology combines low
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Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Ind
Human embryonic stem cells efficiently differentiate blood vessels, which allows
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Phylogenomic Analysis by Chromosome Sorting and Painting
Chromosome sorting by flow cytometry is the principle source of chromosome-speci
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Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Hel
Mitochondrial DNA (mtDNA) maintenance can be and has been studied in a wide vari
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High Cell-Density Fermentation
The purpose of this chapter is to provide sufficient instruction for the reader
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