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肝中DNA的分离与鉴定原理
细胞内的核酸多以核蛋白的形式存在，其中脱氧核糖核蛋白主要存在于细胞核中，核糖核蛋白主要存在于细胞质中。这两类核蛋白在0.14mol／L氯化钠溶液中的溶解度相差很
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Freeze Squeeze PCR Product Purification from Agorose Gel
Abstract: The “Freeze squeeze” method is a cost effective and technically simple
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寡核苷酸纯化
Purpose: Purification of oligonucleotides (which have already been purified by r
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Injection DNA Preparation
) Culture 400 mls of bacteria harboring transposon overnight at 37oC.2) Harvest
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DNA isolation extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION
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真菌基因组DNA的提取方法
Fungal Genomic DNA Extraction OverviewHigh throughput of many fungal isolates ca
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细菌基因组DNA提取方法综述
细菌基因组DNA的提取方法综述，提供了5种方法。 1 快速微量提取法 A.取1.5ml菌体培养物于一灭菌Ep管中，12000rpm离心1min, 丢去上清夜，收
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Recovering DNA from agarose gels
Recovering DNA from agarose gelsPaul N. Hengen, Ph.D. (July 14, 1999)Introductio
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间接荧光免疫标记的基础方法
BackgroundThis is the method for indirect immunofluorescence labeling; that is,
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核酸生物合成
一、直接检出病毒核酸 1．核酸杂交（Nucleic acid hybridization） -临床病毒学中报速诊断方法通常是检测标本中的病毒抗原，然而
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丙烯酰胺胶分离DNA
1.Pour a vertical acrylamide gel using TEA buffer.A 4 % non denaturing gel is co
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DEAE membrane吸附法分离与纯化DNA片段
利用离子交换的原理，在低盐情况下使DNA 吸附在带正电荷的滤膜上，再利用含有高浓度盐类的缓冲液将DNA 自膜上溶离下来。仪器用具：Mupid II 迷你电泳槽；
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Sephadex G-50 spun column purificati
Sephadex G-50 spun column purificationSpin column purification can be used to ch
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Isolation of DNA fragments using glass milk (GENE-CLEAN)
1.Excise DNA section from gel into eppendorf tube.2.Add 2-3 volumes of NaI (NaI
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PLASMID MIDI-PREP FROM BACTERIA
PREPARE SOLUTIONS1. Glycerol mix (1 L):Weigh 25 grams (liquid weight) of glycero
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