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DNA isolation extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION
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真菌基因组DNA的提取方法
Fungal Genomic DNA Extraction OverviewHigh throughput of many fungal isolates ca
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细菌基因组DNA提取方法综述
细菌基因组DNA的提取方法综述,提供了5种方法。 1 快速微量提取法 A.取1.5ml菌体培养物于一灭菌Ep管中,12000rpm离心1min, 丢去上清夜,收
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Recovering DNA from agarose gels
Recovering DNA from agarose gelsPaul N. Hengen, Ph.D. (July 14, 1999)Introductio
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间接荧光免疫标记的基础方法
BackgroundThis is the method for indirect immunofluorescence labeling; that is,
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核酸生物合成
一、直接检出病毒核酸 1.核酸杂交(Nucleic acid hybridization) -临床病毒学中报速诊断方法通常是检测标本中的病毒抗原,然而
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丙烯酰胺胶分离DNA
1.Pour a vertical acrylamide gel using TEA buffer.A 4 % non denaturing gel is co
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DEAE membrane吸附法分离与纯化DNA片段
利用离子交换的原理,在低盐情况下使DNA 吸附在带正电荷的滤膜上,再利用含有高浓度盐类的缓冲液将DNA 自膜上溶离下来。仪器用具:Mupid II 迷你电泳槽;
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Sephadex G-50 spun column purificati
Sephadex G-50 spun column purificationSpin column purification can be used to ch
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Isolation of DNA fragments using glass milk (GENE-CLEAN)
1.Excise DNA section from gel into eppendorf tube.2.Add 2-3 volumes of NaI (NaI
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PLASMID MIDI-PREP FROM BACTERIA
PREPARE SOLUTIONS1. Glycerol mix (1 L):Weigh 25 grams (liquid weight) of glycero
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植物基因组DNA的分离纯化及RAPD扩增
1、植物基因组DNA的分离纯化一、实验原理核酸包括DNA、RNA两种分子,在细胞中都是以与蛋白质结合的状态存在。真核生物的染色体DNA是双链线性分子,原核生物的
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DNA precipitation by EtOH
I usually use Linear polyacrylamide as a carrier for DNA precipitation by EtOH.
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Cetyltrimethylammonium bromide (CTAB) Pla
Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liqui
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牙签法小量制备质粒DNA
用牙签从琼脂培养基上挑取的菌落可直接用于制备质粒DNA。所得的闭合环状DNA往往杂质太多,不能作为大多数限制酶的底物。但是,牙签法也有很多用途,例如,它可用来鉴
2024-10-16