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  • DNA isolation extraction

    DNA isolation extraction

    CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION

    2024-10-16
  • 真菌基因组DNA的提取方法

    真菌基因组DNA的提取方法

    Fungal Genomic DNA Extraction OverviewHigh throughput of many fungal isolates ca

    2024-10-16
  • 细菌基因组DNA提取方法综述

    细菌基因组DNA提取方法综述

    细菌基因组DNA的提取方法综述,提供了5种方法。 1 快速微量提取法 A.取1.5ml菌体培养物于一灭菌Ep管中,12000rpm离心1min, 丢去上清夜,收

    2024-10-16
  • Recovering DNA from agarose gels

    Recovering DNA from agarose gels

    Recovering DNA from agarose gelsPaul N. Hengen, Ph.D. (July 14, 1999)Introductio

    2024-10-16
  • 间接荧光免疫标记的基础方法

    间接荧光免疫标记的基础方法

    BackgroundThis is the method for indirect immunofluorescence labeling; that is,

    2024-10-16
  • 核酸生物合成

    核酸生物合成

    一、直接检出病毒核酸   1.核酸杂交(Nucleic acid hybridization) -临床病毒学中报速诊断方法通常是检测标本中的病毒抗原,然而

    2024-10-16
  • 丙烯酰胺胶分离DNA

    丙烯酰胺胶分离DNA

    1.Pour a vertical acrylamide gel using TEA buffer.A 4 % non denaturing gel is co

    2024-10-16
  • DEAE membrane吸附法分离与纯化DNA片段

    DEAE membrane吸附法分离与纯化DNA片段

    利用离子交换的原理,在低盐情况下使DNA 吸附在带正电荷的滤膜上,再利用含有高浓度盐类的缓冲液将DNA 自膜上溶离下来。仪器用具:Mupid II 迷你电泳槽;

    2024-10-16
  • Sephadex G-50 spun column purificati

    Sephadex G-50 spun column purificati

    Sephadex G-50 spun column purificationSpin column purification can be used to ch

    2024-10-16
  • Isolation of DNA fragments using glass milk (GENE-CLEAN)

    Isolation of DNA fragments using glass milk (GENE-CLEAN)

    1.Excise DNA section from gel into eppendorf tube.2.Add 2-3 volumes of NaI (NaI

    2024-10-16
  • PLASMID MIDI-PREP FROM BACTERIA

    PLASMID MIDI-PREP FROM BACTERIA

    PREPARE SOLUTIONS1. Glycerol mix (1 L):Weigh 25 grams (liquid weight) of glycero

    2024-10-16
  • 植物基因组DNA的分离纯化及RAPD扩增

    植物基因组DNA的分离纯化及RAPD扩增

    1、植物基因组DNA的分离纯化一、实验原理核酸包括DNA、RNA两种分子,在细胞中都是以与蛋白质结合的状态存在。真核生物的染色体DNA是双链线性分子,原核生物的

    2024-10-16
  • DNA precipitation by EtOH

    DNA precipitation by EtOH

    I usually use Linear polyacrylamide as a carrier for DNA precipitation by EtOH.

    2024-10-16
  • Cetyltrimethylammonium bromide (CTAB) Pla

    Cetyltrimethylammonium bromide (CTAB) Pla

    Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liqui

    2024-10-16
  • 牙签法小量制备质粒DNA

    牙签法小量制备质粒DNA

    用牙签从琼脂培养基上挑取的菌落可直接用于制备质粒DNA。所得的闭合环状DNA往往杂质太多,不能作为大多数限制酶的底物。但是,牙签法也有很多用途,例如,它可用来鉴

    2024-10-16