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Footprinting
FootprintingFootprinting is a method for determining the exact DNA sequence to w
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How short can be the CpG island-Molecular Biology
Hi there,Is anyone know how short of the CpG island can be? let say if the CG se
2024-10-10 -
Bisulfite modification kits and PCR-DNA Methylation, His
I was wondering if anyone could provide an opinion on the difference in PCR ampl
2024-10-10 -
DNA methylation
DNA methylation is an epigenetic event that affects cell function by altering ge
2024-10-10 -
Bisulfite Modification of DNA
Source: Protocol Online Abstract: Modifying DNA using sodium bisulfite to conver
2024-10-10 -
多囊卵巢综合征病人基因组DNA甲基化免疫沉淀反应分析
多囊卵巢综合征(Polycystic ovary syndrome, PCOS)是一种复杂的、病因不明的疾病，最新研究结果表明，胰岛素耐受(insulin re
2024-10-10 -
Problems in bisulfite sequencing reproducibility-DNA Met
I am having huge problems with bisulfite PCR.Southern analysis indicates that my
2024-10-10 -
甲基化 MSP 特异性 PCR 全程的常见问题与相关控制措施
一、亚硫酸氢钠修饰过程中可能出现的问题及应对措施亚硫酸氢钠修饰DNA的目的是将DNA序列中未甲基化的胞嘧啶完全转化为尿嘧啶，而甲基化的5一甲基胞嘧啶保持不变。影
2024-10-10 -
Promoter Prediction-DNA Methylation, Histone and Chromat
I am planning to perform sodium bisulphite sequencing. Currently, I am trying to
2024-10-10 -
氢盐预处理法检测DNA片段甲基化
1. 基化特异性PCR。甲基化特异性PCR(MSP)是Herman等首先提出的一种检测基因组DNA甲基化水平的常用方法。该法是将DNA经亚硫酸氢钠处理，非甲基化
2024-10-10 -
S1 Nuclease Protection Assay
S1 Nuclease Protection AssayEnd-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5
2024-10-10 -
Nature Genetics最新发表应用甲基化芯片研究白血病文章
表观遗传修饰(epigenetic modification) 是指基于非基因序列改变所致基因表达水平的变化，如DNA甲基化、组蛋白乙酰化和甲基化、RNA相关性
2024-10-10 -
高分辨率熔点分析技术进行冻存组织样本甲基化的检测
1 、前言启动子高度甲基化是恶性肿瘤基因转录中的一种很常见的现象，被视为细胞恶性转化的标志之一。DNA 甲基化分析是一种基因检测工具，用于早期癌症检测、风险评估
2024-10-10 -
How to find the promoter region of a gene for methylatio
This is the URL to my gene of interest.I am trying to estimate the promoter regi
2024-10-10 -
LightCycler通过实时荧光PCR技术进行DNA甲基化分析
DNA甲基化是表观遗传学的重要研究内容之一，它可以在转录水平抑制基因的表达。具体的过程是在胞嘧啶－鸟嘧啶（CpG二核苷酸）的5位碳原子上添加了一个额外的甲基团，
2024-10-10
