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Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
Drug “ligands” that bind G protein-coupled receptors (GPCRs) can either stimulat
2025-01-18 3 -
Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm
The paternal genome in many animal taxa is efficiently packaged into the sperm n
2025-01-18 1 -
Slice Cultures for Study of Microglia
Culture of isolated microglia from dissociated cortical tissue has promoted the
2025-01-18 0 -
Production of Transgenic Mice with Yeast Artificial Chromosomes
The stable introduction of recombinant DNA into the germline of mammals is one o
2025-01-18 1 -
Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation
The process of fatty acid transport across the plasma membrane occurs by several
2025-01-18 3 -
Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
The time and labor required for generating nucleotide sequence information has b
2025-01-18 1 -
Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors
Nature has made extensive use of metals in biological systems. Metals perform a
2025-01-18 0 -
核仁(nucleolus)
核仁是细胞核中一个匀质的球体，由纤维区、颗粒区、核仁染色质、基质等四部分所组成。核仁是真核细胞间期核中最明显的结构。核仁的大小、形状和数目随生物的种类、细胞类型
2025-01-18 7 -
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
The discovery of stem cell populations in the adult central nervous system (CNS)
2025-01-18 2 -
Structural Probing Techniques on Natural Aptamers
RNA sequences fold in a hierarchical manner to form complex structures. This fol
2025-01-18 5 -
Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and
Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin an
2025-01-18 6 -
CCK8实验技巧与改进方案
一、实验技巧①细胞接种优化：在接种细胞时，务必确保细胞悬液混匀，避免细胞沉淀导致每孔中细胞数量不一致。建议使用多通道移液器斜贴着培养板壁加样，减少平行孔间的误差
2025-01-17 14 -
CCK8实验目的
一、CCK-8实验的目的与意义CCK-8实验的主要目的在于快速、准确地评估细胞的增殖和活力。通过测量细胞在特定条件下的生长速度和代谢活性，科研人员能够了解细胞对
2025-01-17 6 -
CCK8实验数据分析
一、数据分析步骤①数据整理与预处理首先，将实验所得的OD值数据整理成表格形式，包括实验组和对照组的数据。对于每个实验组和对照组，需要确保细胞接种密度、培养时间以
2025-01-17 8 -
CCK8实验新手教程
一、实验原理及准备CCK8实验，全称为Cell Counting Kit-8实验，是一种基于WST-8（2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-
2025-01-17 2
