EMSA using ds Oligonucleotides

Solutions 10X Annealing Buffer 200 mM Tris 8.0 200 m l 1M Tris pH 8.0 10 mM EDTA 8.0 20 m l .5M EDTA pH 8.0

500 mM NaCl 100 m l 5M NaCl

280 m l Q

store at room temperature

10X Klenow Buffer

500 mM Tris 7.5 500 m l 1M Tris pH 7.5

100 mM MgCl2 100 m l 1M MgCl 2

10 mM DTT 20 m l 0.5 M DTT

0.5 m g/ m l BSA 50 m l 10 mg/ml BSA (NEB)

330 m l Q

store at -80℃ in 50 m l aliq.

2X Binding Buffer (ref: Gutman et al. GENE 110, 1992 pg 197)

20% glycerol 400 m l 50% glycerol

20 mM Tris 7.5 20 m l 1M Tris pH 7.5

100 mM KCl 100 m l 1M KCl

1 mM DTT 2 m l 0.5 M DTT

478 m l Q

make fresh as needed

Poly dI/dC

Make a 1 m g/ m l stock and store in 100 ul aliq. at -80℃