Cultivation, Spore Production, and Mating

Dictyostelium discoideum proliferates as solitary amoebae, constitutes multicellular structures called fruiting bodies, and mates to form macrocysts depending on environmental conditions. All of these processes can be easily induced in the laboratory. The amoebae are normally cultured with food bacteria, but the strains with mutations in axe loci can proliferate in nutrient media without bacteria. The strains can be stored either as spores or amoebae. Synchronous development of fruiting bodies is initiated by depleting the culture media or food bacteria. Synchronous development of macrocysts is achieved by mixing the cells of heterothallic strains separately cultured in darkness to induce the sexual maturation.