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原位末端转移酶标记技术检测凋亡
（一）原理 凋亡细胞是由于内源性核酸内切酶的激活后，将DNA 切割成许多双链DNA 片段以及高分子量DNA 单链断裂点（缺口），暴露出大量3- 羟基末端，如用末
2025-03-07 40 -
Cell Quantification
Aim For the majority of manipulations using cell cultures, such as transfections
2025-03-07 23 -
细胞培养实验中常用器材和试剂的配制
相关专题之前讲述了细胞培养 的常用的两个工具即细胞培养瓶和细胞培养板，这次再来说下细胞培养常用的器材和试剂，主要讲述了细胞培养常用试剂的配制。细胞培养常用的器材
2025-03-07 33 -
凝集素(lectin)
凝集素是动物细胞和植物细胞都能够合成和分泌的、能与糖结合的蛋白质,在细胞识别和粘着反应中起重要作用,主要是促进细胞间的粘着。凝集素具有一个以上同糖结合的位点，因
2025-03-07 26 -
M6P受体蛋白(M6P receptor protein)
M6P受体蛋白是反面高尔基网络上的膜整合蛋白, 能够识别溶酶体水解酶上的M6P信号并与之结合, 从而将溶酶体的酶蛋白分选出来，然后通过出芽的方式将溶酶体的酶蛋白
2025-03-07 49 -
Gas Chromatography-Mass Spectrometry Method for the Determination of Methadone and 2-Ethylidene-1,5-
Methadone is a synthetic opioid used to relieve pain, treat opioid withdrawal, a
2025-03-07 36 -
Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction
Fluorescence correlation spectroscopy (Bacia and Schwille (2007) Nat. Protoc. 2
2025-03-07 20 -
Post-translational Modification of p53 by Ubiquitin
Post-translational modification of p53 by ubiquitin resides in the center of a f
2025-03-07 23 -
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand brea
2025-03-07 24 -
293-T细胞培养实验中注意事项
相关专题培养293细胞 应注意以下几个方面：1.用1640加10%胎牛血清，有的种系用DMEM .血清要求质量较高，在普通血清中细胞生长不太好。1640用双蒸水
2025-03-07 29 -
Ribonuclease Protection Analysis of Gene Expression in Xenopus
When characterizing the developmental expression of a novel gene, or when examin
2025-03-07 25 -
Observational Study Design
Much can be learned about a process by observing changes over time or by compari
2025-03-07 25 -
Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices
Research in cell biology often is based in tissue culturing cells on artificial
2025-03-07 22 -
Immobilization of Enzymes on Thermo-Responsive Polymers
Immobilization of enzymes on supports is a valuable technique for increasing tot
2025-03-07 24 -
Analysis of Rho GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
Rho GTPases are important regulators of the formation of focal adhesions and foc
2025-03-07 21
