Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

  • 细胞因子的检测方法

    细胞因子的检测方法

    细胞因子(cytokine)是由细胞分泌的具有生物活性的小分子蛋白物质的统称。在免疫应答过程中,细胞因子在免疫调节、炎症应答、肿瘤转移等生理和病理过程中起重要作

    2025-03-20 78
  • Idiotype Gene Rescue in Follicular Lymphoma

    Idiotype Gene Rescue in Follicular Lymphoma

    Beyond the morphological, immunophenotypic, and genetic information used for the

    2025-03-20 64
  • Clinical Protocol for p53 Gene Therapy for Liver Tumors

    Clinical Protocol for p53 Gene Therapy for Liver Tumors

    This chapter is intended to help other workers with the preparation of human gen

    2025-03-20 48
  • Tailoring Natural Effector Functions: Antibody Engineering Beyond Humanization

    Tailoring Natural Effector Functions: Antibody Engineering Beyond Humanization

    A large number of new biological drugs in clinical development from the biotechn

    2025-03-20 50
  • 抑制大肠癌向肝脏转移的新方法

    抑制大肠癌向肝脏转移的新方法

    日本研究人员在新一期美国《国家科学院学报》网络版上报告说,通过阻止骨髓细胞内的某种蛋白质与激素结合,可防止大肠癌向肝脏转移。京都大学遗传药理学教授武藤诚率领的研

    2025-03-20 47
  • Molecular Characterization of Inflammation-Induced JNK/c-Jun Signaling Pathway in Connection with Tu

    Molecular Characterization of Inflammation-Induced JNK/c-Jun Signaling Pathway in Connection with Tu

    Tumor cells recruit inflammatory cells to the tumor site and transform them into

    2025-03-20 58
  • Single-Domain VH Antibody Fragments from a Phage Display Library

    Single-Domain VH Antibody Fragments from a Phage Display Library

    The interaction between antigens and antibodies can be viewed as a protein engin

    2025-03-20 45
  • Galectin-3 Binding and Metastasis

    Galectin-3 Binding and Metastasis

    Galectin-3 is a member of a family of carbohydrate-binding proteins. It is prese

    2025-03-20 62
  • 细胞因子和重组蛋白溶解必读(一)

    细胞因子和重组蛋白溶解必读(一)

    我们知道,PeproTech的所有细胞因子和蛋白均为冻干粉,这使得运输非常便捷,只要常温即可。而且,细胞因子和蛋白冻干粉非常稳定,在-20℃或-80℃条件下可保

    2025-03-20 90
  • 转染细胞不表达怎么办

    转染细胞不表达怎么办

    问:最近做细胞转染,预筛选稳定克隆,载体为pEGFP-N1,pIERS现在是平行做几个基因,转然后,只有空载体和一个基因可以看到绿色荧光,其余均没有,这会是什么

    2025-03-20 65
  • 萤光素酶载体PGL3转染细胞要不要加G418

    萤光素酶载体PGL3转染细胞要不要加G418

    问:最近要做萤光素酶载体PGL3转染细胞,PGL3载体上面带有neo的抗性标记,有一个老师和我说要培养基里面要加G418筛选,但我查了一些PGL3载体转染的文献

    2025-03-20 49
  • g418如何配制

    g418如何配制

    问:最近要做稳定转染了,但不知道如何配制G418,有一些细节想问问大家,我打算用PBS来配,我想问问是用高压过的PBS来配,还是用未高压的PBS配,配完了是不是

    2025-03-20 68
  • 细胞接种密度

    细胞接种密度

    问:最近的一个实验困扰我许久,就是关于给药剂量的问题,具体如下: 我在培养瓶中按1*10E6密度观察了心肌细胞的搏动和药物的干预情况,接下来想做MTT,需要用9

    2025-03-20 84
  • 质体转染293细胞后,细胞漂浮死亡?

    质体转染293细胞后,细胞漂浮死亡?

    问:这几天做293细胞的脂质体转染,为腺病毒的包装阶段.用最小的培养瓶(好像是25ml的),加入脂质体10μl,pacI酶切纯化物8μg 进行转染.细胞最密处为

    2025-03-20 57
  • Intercellular Adhesion Molecules (ICAM-1, VCAM-1, and LFA-1) in Adenocarcinoma of Lung: Technical As

    Intercellular Adhesion Molecules (ICAM-1, VCAM-1, and LFA-1) in Adenocarcinoma of Lung: Technical As

    Cell adhesion molecules (CAMs) such as intercellular adhesion molecule (ICAM-1),

    2025-03-20 56