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Chimeric G Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors
High throughput calcium mobilization assays are extensively used for pharmacolog
2025-04-11 5 -
Instrumentation for Liquid Chromatography
High performance liquid chromatography (HPLC) is one of the most rapidly growing
2025-04-11 5 -
Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins
Heterotrimeric G proteins are composed of α, β, and γ subunits. G proteins can b
2025-04-11 3 -
Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties
Heterobifunctional crosslinking reagents are small molecular weight chemicals co
2025-04-11 4 -
Immunological assays for chemokine detection in in-vitro culture of CNS cells
Herein we review the various methods currently in use for determining the expres
2025-04-11 7 -
Serum-free cryopreservation of five mammalian cell lines in either a pelleted or suspended state
Herein we have explored two practical aspects of cryopreserving cultured mammali
2025-04-11 4 -
Evaluating Amyloid Beta (A) 1-40 Degradation by Capillary Electrophoresis
Here, we describe a capillary electrophoresis method for evaluating proteolysis
2025-04-11 9 -
In Vitro Assays of Orphan Glycosyltransferases and Their Application to Identify Notch Xylosyltransf
Here we describe a systematic approach to determine the activity of putative gly
2025-04-11 4 -
Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B
Hepatic lipase (HL) is necessary for efficient clearance of triglyceride-rich li
2025-04-11 4 -
Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
Heat-shock transcription factors (Hsfs) regulate transcription of heat-shock pro
2025-04-11 6 -
Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase
Hapten-protein conjugates are essential in many immunochemical assays and in par
2025-04-11 4 -
Screening Effective Target Sites on mRNA: A Ribozyme Library Approach
Hammerhead ribozymes have been extensively used as RNA-inactivating agents for t
2025-04-11 7 -
Design of Hybridizing Arms in Hammerhead Ribozymes
Hammerhead ribozymes as typically used in their trans -cleaving form are defined
2025-04-11 4 -
Synthesis, purification and crystallization of guanine-rich RNA oligonucleotides
Guanine-rich RNA oligonucleotides display many novel structural motifs in recent
2025-04-11 4 -
Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction
Group I ribozymes are naturally occurring catalytic RNAs that are able to excise
2025-04-11 6
