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叶绿体被膜(membrane envelope)
叶绿体的外膜和内膜合称为被膜。叶绿体的外膜和内膜都是连续的单位膜, 每层膜的厚度为6～8nm, 内外两膜间有10～20nm宽的间隙, 称为膜间隙。与线粒体不同的
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光系统(photosystem)
进行光吸收的功能单位称为光系统, 是由叶绿素、类胡萝卜素、脂和蛋白质组成的复合物。每一个光系统含有两个主要成分∶捕光复合物(light -harvesting
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内共生学说(endosymbiont hypothesis)
关于线粒体起源的一种学说。认为线粒体来源于细菌，即细菌被真核生物吞噬后,在长期的共生过程中,通过演变,形成了线粒体。该学说认为:线粒体祖先原线粒体(一种可进行三
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培养细胞染色体显色法
培养细胞染色体显示法 1） 传代培养细胞染色体显示法1． 培养细胞：取处于指数生长期、用较大瓶皿培养的、 80 ％～ 90 ％汇合单层培养细胞。2． 加秋水仙
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The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
The use of affinity tags and especially histidine tags (His-tags) has become wid
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Simplified Calcium Phosphate Coprecipitation
Materials 2x HEPES: 8 g NaCl, 0.105g NaHPO4 , 6.5 g HEPES, H2O to 500 mL, pH 6.9
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肽酰转移酶(peptidyl transferase)
肽酰转移酶是催化肽键形成的酶。在蛋白质合成过程中，它催化核糖体A位tRNA上末端氨基酸的氨基与P位肽酰-tRNA上氨基酸的羧基间形成肽键。其结果,使A位的氨酰-
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MudPIT Analysis: Application to Human Heart Tissue
Although two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been u
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Assays to Measure Stress-Activated MAPK Activity
Mitogen-activated protein kinases (MAPKs) are activated by a wide variety of cel
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Classical Human Epidermal Keratinocyte Cell Culture
It has been more than 30 years since the serial cultivation of human keratinocyt
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MTS检测细胞生长
1． 培养 IL-2 依赖细胞株 CTLL-2 细胞或 HT-2 细胞（检测 IL-2 ），或者 IL-3 依赖细胞株 FDC-P1 细胞或 FL5.12 细胞
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Measurements of White Adipose Tissue Metabolism by Microdialysis Technique
The microdialysis method was introduced 25 yr ago to measure neurotransmitter co
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Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis
Liquid chromatography (LC) can be combined with matrix-assisted laser-desorption
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In Vitro Matrigel Angiogenesis Assays
A variety of in vivo and in vitro methods have been used to study angiogenesis,
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Locked Nucleic Acid Aptamers
The aptamer technology has been introduced in the early 1990s. With this techniq
