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细胞培养实验中常用器材和试剂的配制
相关专题之前讲述了细胞培养 的常用的两个工具即细胞培养瓶和细胞培养板,这次再来说下细胞培养常用的器材和试剂,主要讲述了细胞培养常用试剂的配制。细胞培养常用的器材
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凝集素(lectin)
凝集素是动物细胞和植物细胞都能够合成和分泌的、能与糖结合的蛋白质,在细胞识别和粘着反应中起重要作用,主要是促进细胞间的粘着。凝集素具有一个以上同糖结合的位点,因
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M6P受体蛋白(M6P receptor protein)
M6P受体蛋白是反面高尔基网络上的膜整合蛋白, 能够识别溶酶体水解酶上的M6P信号并与之结合, 从而将溶酶体的酶蛋白分选出来,然后通过出芽的方式将溶酶体的酶蛋白
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Gas Chromatography-Mass Spectrometry Method for the Determination of Methadone and 2-Ethylidene-1,5-
Methadone is a synthetic opioid used to relieve pain, treat opioid withdrawal, a
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Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction
Fluorescence correlation spectroscopy (Bacia and Schwille (2007) Nat. Protoc. 2
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Post-translational Modification of p53 by Ubiquitin
Post-translational modification of p53 by ubiquitin resides in the center of a f
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Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand brea
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293-T细胞培养实验中注意事项
相关专题培养293细胞 应注意以下几个方面:1.用1640加10%胎牛血清,有的种系用DMEM .血清要求质量较高,在普通血清中细胞生长不太好。1640用双蒸水
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Ribonuclease Protection Analysis of Gene Expression in Xenopus
When characterizing the developmental expression of a novel gene, or when examin
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Observational Study Design
Much can be learned about a process by observing changes over time or by compari
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Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices
Research in cell biology often is based in tissue culturing cells on artificial
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Immobilization of Enzymes on Thermo-Responsive Polymers
Immobilization of enzymes on supports is a valuable technique for increasing tot
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Analysis of Rho GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
Rho GTPases are important regulators of the formation of focal adhesions and foc
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Maintenance of Cell Culture
Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci Don
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Cell Lineage Analysis: X-Inactivation Mosaics
In female mammals, one of the two X chromosomes in each and every embryonic cell