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Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transi
The mitochondrial genome represents a target for exogenous and endogenous damage
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Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Primin
Specific cytotoxic T-lymphocytes (CTL) are important in the protection against v
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Cell Grafting and Fate Mapping of the Early-Somite-Stage Mouse Embryo
The development of techniques for culturing postimplantation mouse embryos has o
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Renal and Cardiac Na+K+-ATPase and Aconitase in a Rat Model of Fetal Programming
Fetal programming of adult disease is an area of research that has gained consid
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酵母菌落直接质粒转化法
酵母菌落直接质粒转化法 1． 用牙签从平板中挑取单菌落（直径 2 ～ 3mm ），转移到 1.5ml 的无菌离心管中。2． 将 10 μ l 载体 DNA （
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Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice
Gene inactivation has become the gold standard for determining gene function in
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G418筛选慢病毒感染稳定细胞株
用慢病毒筛选稳定细胞株，以G418筛选方法为例，筛选出稳定整合Neomycin抗性基因的细胞系。A 、 G418 抗生素最优浓度筛选每种细胞对抗生素的敏感性不同
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Colony Forming Unit Assays for MSCs
MSCs are the plastic adherent adult stem/progenitor cells from bone marrow origi
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低毒性X-tremeGENE DNA转染检测
相关专题 总有一种转染方法适合你前言X-tremeGENE DNA Transfection Reagent是一种非脂质体、多组分转染 试剂，已被证明可有效转染
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Biochemical Isolation of mtDNA Nucleoids from Animal Cells
Mitochondrial DNA (mtDNA) in animal cells is organized into clusters of 5–7 geno
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向导RNA(guide RNA, gRNAs)
引导RNA编辑的RNA分子,长度大约是60～80个核苷酸,是由单独的基因转录的,具有3＇寡聚U的尾巴,中间有一段与被编辑mRNA精确互补的序列,5＇端是一个锚定
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受体( receptor)
受体在细胞生物学中是一个很泛的概念,意指任何能够同激素、神经递质、药物或细胞内的信号分子结合并能引起细胞功能变化的生物大分子。在细胞通讯中,由信号传导细胞送出的
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啤酒酵母菌营养缺陷型菌株的筛选
相关专题 一、实验原理利用化学诱变剂诱发突变 是遗传学研究和育种工作的常用手段。化学诱变剂按其诱变机理一般可分为三类：（1）通过掺入DNA分子引起突变，（2）
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微粒体(microsomes)
微粒体是细胞被匀浆破碎时, 内膜系统的膜结构破裂后自己重新封闭起来的小囊泡(主要是内质网和高尔基体), 这些小囊泡的直径大约100 nm左右, 是异质性的集合体
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Capillary Electrophoresis With Laser-Induced Fluorescence: Environmental Applications
Capillary electrophoresis (CE), especially free-zone CE, offers a relatively sim
