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人工染色体(artificial chromosome)
基于ARS、CEN、TEL是线性染色体稳定的功能序列,人们利用这些序列构建载体, 重组后的DNA以线性状态存在, 这样不仅稳定,而且大大提高了插入外源基因的能力
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Immobilization of Whole Cells by Chemical Vapor Deposition of Silica
Effective entrapment of whole bacterial cells onto solid-phase materials can sig
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Isolation and Enrichment of Stro-1 Immunoselected Mesenchymal Stem Cells from Adult Human Bone Marro
The availability of mesenchymal stem cells (MSCs) or skeletal stem cells (SSCs)
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Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis
There is an increasing evidence that uncoupling protein-2 (UCP2), a recently ide
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Diagonal Electrophoresis for Detection of Protein Disulphide Bridges
A state of oxidative stress (OS) can occur when there is an imbalance between th
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实用哺乳动物细胞培养手册
相关专题细胞培养 基本概念 细胞培养是指从体内组织取出细胞在体外模拟体内环境下,使其生长繁殖,并维持其结构和功能的一种培养技术。细胞培养的培养物可以是单个细胞,
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Cell-Free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts
Cholesterol-rich microdomains present on the plasma membrane appear to play an i
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核糖核酸聚合酶(RNA polymerase)
催化RNA合成的酶。主要指DNA指导的RNA聚合酶,即以DNA为模板,以4种核苷三磷酸(ATP,GTP,CTP和UTP)为底物,从5′到3′合成RNA的酶。其催
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FISH With and Without COT1 DNA
Complex FISH probes comprising large spans of genomic DNA always contain a high
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异固缩(heteropythosis)
细胞分裂时, 核内染色质要凝缩成染色体结构,对碱性染料着色很深,一旦脱离分裂期, 染色体去凝集成松散状态, 此时染色着色力减弱。但是,有些染色体或其片段的凝缩周
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Urine Proteins
Urine protein electrophoresis has been used primarily to confirm the presence or
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Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Cho
Differentiation procedures leading to osteogenic and chondrogenic differentiatio
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Design and Analysis of Experiments
This chapter is primarily devoted to experiments that compare 2 treatments with
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Bioinformatics Strategies for the Analysis of Lipids
Owing to their importance in cellular physiology and pathology as well as to rec
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Computer-Aided Sperm Analysis (CASA) of Sperm Motility and Hyperactivation
Progressive motility is a vital functional characteristic of ejaculated human sp