-
Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry
Progression of cells through successive phases and checkpoints of the cell cycle
-
Extrachromosomal Assay for DNA Double-Strand Break Repair
DNA double-strand break (DSB) repair in mammalian cells has been demonstrated to
-
Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Indiv
In order to restore or to introduce a gene function integrating viral vector sys
-
Direct Sequencing of PCR Products Using Chemiluminescent Detection
The following protocol describes a method that can be used for the direct sequen
-
Live Imaging of LFA-1-Dependent T-Cell Motility and Stop Signals
T-cell motility is critical for leukocyte trafficking both in normal host defens
-
Computer-Assisted Interpretation of Triacylglycerols Mass Spectra
Triacylglycerols (TGs) are principal components of vegetable oils and animal fat
-
胶原(collagen)
胶原是细胞外最重要的水不溶性纤维蛋白, 是构成细胞外基质的骨架。胶原在细胞外基质中形成半晶体的纤维,给细胞提供抗张力和弹性,并在细胞的迁移和发育中起作用。胶原在
-
Group Migration and Signal Relay in Dictyostelium
The ability of cells to migrate directionally in gradients of chemoattractant is
-
Purifying the Hedgehog Protein and its Variants
The purification of recombinant versions of the N-terminal signaling fragment of
-
Quantitative Visualization of Autophagy Induction by mTOR Inhibitors
Autophagy is a catabolic pathway that degrades bulk cytosol in lysosomal compart
-
Studying Early Hematopoiesis Using Avian Blastoderm Cultures
The process of hematopoiesis occurs very early during embryogenesis, with the ap
-
125I-UdR致C6胶质瘤细胞损伤的模型
一、材料:125I-UdR购自中科院北京原子能所,放化纯度>95%。脱氧腺苷(AdR)、脱氧鸟苷(GdR)、脱氧胞苷(CdR)、脱氧尿苷(UdR)均为Si
-
Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
RNA interference is widely used for loss-of-function studies in mammalian cells.
-
Cationic α-Helical Peptides for Gene Delivery into Cells
Development of nonviral gene transfer techniques has progressed, particularly th
-
Lipoplex Formation Using Liposomes Prepared by Ethanol Injection
Cationic liposomes composed of 3β-[N -(N� N� –dimethylaminoethane)carbamoyl] cho