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Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Anal
The completion of the human genome sequence and availability of cDNA microarray
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Collagen Invasion Assay
Invasion occurs when invasion promoter molecules outbalance the function of inva
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用于细胞分析的机器视觉系统简介
1996年,美国盐湖域的阿鲁普实验室遇到一个问题。该实验室从事帕帕尼科拉乌涂片的筛分工作以便找出宫颈癌症兆。当涂片上有少量异常细胞存在而又未被探测出来时,他们会
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Use of Splicing Reporter Minigene Assay to Evaluate the Effect on Splicing of Unclassified Genetic V
The interpretation of the numerous sequence variants of unknown biological and c
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G-Rich Oligonucleotides for Cancer Treatment
Oligonucleotides with guanosine-rich (G-rich) sequences often have unusual physi
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Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas
Engineering of antibodies specific for tumor-associated antigens provides great
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软琼脂克隆形成实验
原理:细胞接种存活率只表示接种细胞后贴壁的细胞数,但贴壁后的细胞不一定每个都能增殖和形成克隆。而形成克隆的细胞必为贴壁和有增殖活力的细胞。克隆形成率反映细胞群体
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真核生物翻译的调控
原核生物基因表达的调控主要在转录水平上进行,而真核生物由于RNA较为稳定,所以除了存在转录水平的调控以外,在翻译水平上也进行各种形式的调控。在蛋白质生物合成的起
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d-Glucaric Acid as a Prospective Tumor Marker
d -Glucaric acid (GA) 1s a natural, apparently nontoxic compound produced in sma
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Diet, Epigenetics, and Cancer
Cancer encompasses a highly heterogeneous group of diseases. It has been thought
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Interaction of p53 with Cellular Proteins
Cellular proteins that interact with p53 play a major role in both positive and
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有限稀释法
有限稀释法的程序①制备饲养细胞悬液②转染了重组质粒后的细胞计数,并调细胞数在1~5103/ml③取130个细胞放入6.5ml含饲养细胞完全培养液,即20个细胞/
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Enhanced Permeability and Retention (EPR) Effect for Anticancer Nanomedicine Drug Targeting
Effective cancer therapy remains one of the most challenging tasks to the scient
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Multicolor Spectral Karyotyping in Multiple Myeloma
Multiple myeloma is a plasma cell disorder characterized at the cytogenetic leve
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luciferase检测的标准品问题
荧光素酶作为一种报告基因要在检测时比较其相对发光强度值,就要在转染时引入另外一个报告基因进行转染条件、效率的标准化归一。Promega公司在这方面有双荧光素酶检