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FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms
Planarians provide a relatively simple model system in which to study stem cell
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Culture Systems: Single Step
Culture media to support development of zygotes to the blastocyst stage is based
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Fractionation of the Coxiella burnetii Parasitophorous Vacuole
Coxiella burnetii is a bacterial obligate intracellular pathogen that replicates
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Designing, Testing, and Validating a Microarray for Stem Cell Characterization
Microarray technology is a powerful tool that allows for simultaneous assessment
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Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (SnyderRobinson Syndrome)
Polyamines, small positively charged molecules, are vital for cell proliferation
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基因扩增(gene amplification)
细胞内选择性复制DNA, 产生大量的拷贝。如两栖类卵母细胞在发育的早期,rRNA基因的数量扩增到1000多倍。基因扩增是通过形成几千个核进行的,每个核里含有几百
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Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2
Many cell cycle regulatory proteins have been shown to be able to regulate cell
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Quantitative Analysis of JAK Binding Using Isothermal Titration Calorimetry and Surface Plasmon Reso
Janus Kinases (JAKs) are the key effector kinases that initiate intracellular si
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Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells
Islet transplantation is a widely accepted and practiced cell replacement therap
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Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Open
This chapter describes a method of assaying rubidium (Rb+ ) efflux as a measure
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ECM Degradation Assays for Analyzing Local Cell Invasion
Proteolytic degradation of extracellular matrix (ECM) is a critical step during
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Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis
Blue native gel electrophoresis (BNGE) is a powerful tool for analyzing native p
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Managing Apheresis Complications During the Hematopoietic Stem Cell Collection
Adverse reactions may occur during the Hematopoietic Progenitor Cells via Aphere
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Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
The functional and molecular characterization of mouse epidermal stem cells (SC)
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A Historical Perspective of the EGF Receptor and Related Systems
Since the isolation of epidermal growth factor (EGF) from mouse submaxillary gla