-
Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-T
Membrane proteomic analysis is of considerable interest due to the role of recep
-
核纤层(lamina)
在与核质相邻的核膜内表面有一层厚30～160nm 网络状蛋白质, 叫核纤层, 对核被膜起支撑作用。核纤层由3种分子量为6～7万道尔顿的多肽亚单位α、β、γ所组成
-
磷脂交换蛋白(phospholipid exchang proteins, PEP)
PEP是一种水溶性的载体蛋白,可以在不同的膜结合细胞器之间转移磷脂。转移的过程是: PEP首先与磷脂分子结合, 形成水溶性的复合物进入细胞质基质中, 通过自由扩
-
Quantitative X-ray Imaging of Rodent Bone by Faxitron
This chapter describes the use of digital micro-radiography with the Faxitron ma
-
Mechanical Properties of Bone Ex Vivo
The primary functions of bone are to do with support and protection – mechanical
-
Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis
Identification and characterization of proteins are ultimately the goal in prote
-
Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Synchronized populations of large numbers of cells can be obtained by centrifuga
-
Freezing and Thawing Cultured Cells
Freezing Cells: 1. Trypsinize cells and harvest in the normal way. 2. Count a 20
-
The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells
In this chapter, we describe a method to isolate and to expand multipotent adipo
-
In Vitro Culture of Rodent Embryos During the Early Postimplantation Period
Mammalian embryos grow within the uterus. This inaccessibility makes mammalian e
-
Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
Lentiviral vectors based upon human immunodeficiency type I (HIV) are increasing
-
Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases
G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs) are tra
-
Imaging Ca2+ Signals in Xenopus Oocytes
Xenopus oocytes have become a favored preparation in which to study the spatiote
-
Identification of MetaboliteRiboswitch Interactions Using Nucleotide Analog Interference Mapping and
Riboswitches are RNA elements capable of modulating gene expression through inte
-
Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem
Deciphering the differentiation pathway of embryonic stem (ES) cells is a challe
