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Production of Zebrafish Germline Chimeras from Cultured Cells
Because of its many favorable characteristics, the zebrafish has become a popula
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Cloning and Mutational Analysis of the Shc-Phosphotyrosine Interaction/Phosphotyrosine-Binding Domai
The interaction between tyrosme phosphorylated growth-factor receptors and Src H
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Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells
The chemical mutagenesis of mouse embryonic stem (ES) cells is an approach compl
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Electron Spectroscopic Imaging of the Nuclear Landscape
Our understanding of sub-nuclear organisation is largely based on fluorescence a
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A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity
A key to understanding cytoskeletal mechanisms of eukaryotic cells is found in t
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Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques
Microtubule organization and dynamics are controlled by a large set of cellular
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StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data
StemBase is a database of gene expression data obtained from stem cells and deri
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Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
Following the initial discovery of split genes in 1977, it took several years be
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Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Ad
MicroRNAs (miRNA) are single-stranded RNA molecules of 21–23 nucleotides in leng
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Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization
DNA methylation is an important epigenetic modification that regulates developme
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Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging
For certain applications, particularly experiments involving high-resolution ima
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亮氨酸拉链基元(leucine zipper motif)
这类蛋白质都是含有4个或5个亮氨酸残基, 彼此之间精确的相距7个氨基酸残基。这样, 在α螺旋的每一个侧面就出现一个Leu, 这些Leu排成一排, 两个蛋白质分子
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Detection of Apoptosis in Drosophila
Drosophila has unique genetic and cell biological advantages as a model system f
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Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis
HOX homeodomain proteins are thought to be master developmental regulators of ti
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The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels
Over the last decade, real-time-quantitative PCR (RT-qPCR) analysis has become t
