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TP53 Mutation Detection by SSCP and Sequencing
The TP53 tumor suppressor gene on chromosome 17p13.1 contains 11 exons and encod
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基因细胞内导入技术
一、基本概念（一）基因导入(或基因转导)将外源性基因(或基因组DNA)采用分子生物学技术人工地导入细胞，观察它在细胞中的表达，研究其生物学特性和功能，这种把基因
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Surface-Epitope Masking (SEM): An Immunological Subtraction Approach for Developing Monoclonal Antib
An immunological subtraction approach, surface-epitope masking (SEM), is describ
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跨越世纪的生命赞歌（第二部分）
863计划生物技术领域15周年回眸。影片回顾了863领域生物技术的起步、开展与成果，成果主要介绍了两系法杂交水稻、转基因抗虫棉、抗病小麦、人类基因组计划、生物技
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用EBV转染淋巴细胞建立永生化细胞株的方法
一、 试剂1、 淋巴细胞分离液：避光4度保存，用前在37度水浴中加温。整个分离过程中，温度应控制在8-28度，温度过高或过低均影响分离质量。2、 全培养基（用前
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细胞房工作守则及注意事项
一、 常规操作1. 穿着专用实验服。自己的白大衣或厚外套，可脱在门外的衣帽架上。 2. 穿着专用拖鞋。在缓冲间换下自己的鞋子，尽量避免在缓冲间走动、停留。
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Telomerase Assay in Renal Cancer
Telomeres are repeating sequences located at each end of eukaryotic chromosomes.
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Gold Nanorods: Multifunctional Agents for Cancer Imaging and Therapy
Gold nanorods (GNRs) are strongly absorbing at near-infrared (NIR) frequencies a
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无白血病抑制因子条件下利用SC1（pluripotin）维持小鼠胚胎干细胞的自我更新
小鼠胚胎干细胞常规培养时需要用到白血病抑制因子（LIF），但是LIF非常昂贵。本视频将展示一种很实惠的替代方法，即以小分子SC1代替LIF维持小鼠胚胎干细胞自我
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Immunohistochemical Localization of TNFα and Its Receptors in the Rodent Central Nervous System
The protocols for immunohistochemical localization of tumor necrosis factor α (T
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Epigenetics in Myeloid Malignancies
Myeloid hematological malignancies are among the epigenetically best characteriz
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Detection of ras Gene Mutations Using Oligonucleotide Ligation Technology
The human ras genes (H-, K-, and N-rus ) are members of a superfamily of low-mol
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p53-Induced Gene Expression Analysis
Researchers in the p53 field have successfully used many high-throughput screeni
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N-Glycosylation Analysis by HPAEC-PAD and Mass Spectrometry
Changes in protein glycosylation are a hallmark of most types of cancer includin
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细胞核内显微注射DNA至游离成年哺乳动物神经细胞
详细描述了在细胞核内显微注射法注射cDNA至游离成年哺乳动物交感神经神经元的过程。 0:00 细胞核内显微注射DNA至游离成年哺乳动物神经细胞0 0:55 引言
