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巨噬细胞的分离与纯化实验方法
相关专题 一)从小鼠、豚鼠或家兔腹腔中分离巨噬细胞1.取6周左右的小鼠(或600克左右的豚鼠，或3公斤左右的家兔)，剃去腹部的毛并消毒。腹腔注射1ml(豚鼠2
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Fluorescein labeling of proteins
Fluorescein labeling of proteinsThis method uses carboxyfluorescein succinimidyl
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FISH Banding Techniques
During the last decade, numerous chromosome banding techniques based on FISH hav
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Blastocyst Culture for Deriving Human Embryonic Stem Cells
Success in producing a human embryonic stem cell line depends largely on the qua
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Assessing Cell Size and Cell Cycle Regulation in Cells with Altered TOR Activity
Target of rapamycin (TOR) regulates the growth of cells and organisms. Numerous
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Modeling miRNA Regulation in Cancer Signaling Systems: miR-34a Regulation of the p53/Sirt1 Signaling
MicroRNAs (miRNAs) are a family of small regulatory RNAs whose function is to re
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Aptamers Targeting RNA Molecules
Oligonucleotides complementary to RNA sequences interact poorly with folded targ
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Internal Standards in Differentiating Embryonic Stem Cells In Vitro
Embryonic stem (ES) cell lines are important for use in developmental biology st
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Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by Using Small Molecules
The derivation of mouse embryonic stem cells (mESCs) from the inner cell mass (I
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Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C1
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Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection
In traditional bone marrow transplantation, the majority of peripherally introdu
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Isolation and Culture of Human Umbilical Vein Endothelial Cells
The vascular endothelium was, in the past, considered to be relatively pas- sive
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Nuclear Reprogramming by Cell Fusion
The use of cell fusion to study exchange of information at the molecular level b
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Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template
Polymerase chain reaction (PCR) is a powerful and efficient method allowing enzy
