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Cryopreservation of Shoot-Tips and Meristems
The ability to regenerate whole plants from cryopreserved, meristematic shoot ti
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Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells
The introduction of restriction endonucleases into mammalian cells in culture pr
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Generation and Use of Epitope-Tagged Receptors
Epitope tagging of a receptor involves introducing a defined amino acid sequence
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Cystic Fibrosis Airway Epithelial Cell Culture
Cystic fibrosis (CF) is the most frequent (incidence around l/2500 live births)
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Characterization of B-Cell Maturation in the Peripheral Immune System
In the periphery different populations of B cells can be identified, correspondi
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Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
Drug “ligands” that bind G protein-coupled receptors (GPCRs) can either stimulat
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Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm
The paternal genome in many animal taxa is efficiently packaged into the sperm n
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Slice Cultures for Study of Microglia
Culture of isolated microglia from dissociated cortical tissue has promoted the
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Production of Transgenic Mice with Yeast Artificial Chromosomes
The stable introduction of recombinant DNA into the germline of mammals is one o
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Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation
The process of fatty acid transport across the plasma membrane occurs by several
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Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
The time and labor required for generating nucleotide sequence information has b
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Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors
Nature has made extensive use of metals in biological systems. Metals perform a
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核仁(nucleolus)
核仁是细胞核中一个匀质的球体，由纤维区、颗粒区、核仁染色质、基质等四部分所组成。核仁是真核细胞间期核中最明显的结构。核仁的大小、形状和数目随生物的种类、细胞类型
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Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
The discovery of stem cell populations in the adult central nervous system (CNS)
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Structural Probing Techniques on Natural Aptamers
RNA sequences fold in a hierarchical manner to form complex structures. This fol
