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Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells
The ability to stably introduce genetic material into primate embryonic stem (ES
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Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin-16
The integral tight junction protein Claudin-16 (Cldn16) is predominantly express
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Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage
Many research groups are engaged in using human pluripotent stem cells (hPSCs) t
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Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies
Two techniques have been described that use the polymerase chain reaction (PCR)
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With the development of the technique of autoradiography by Howard and Pelc (16 ), the mammalian cel
Two techniques have been described that use the polymerase chain reaction (PCR)
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Cell Synchrony Techniques: A Comparison of Methods
With the development of the technique of autoradiography by Howard and Pelc (16
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Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structur
The collagens, proteoglycans, and glycoproteins of the extracellular matrix (ECM
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Gene Dosage in Capillary Electrophoresis: Prenatal Diagnosis of Downs Syndrome and Rh D/d Genotyping
The first instrumentation for capillary zone electrophoresis (CZE) became availa
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Highly Efficient Directed Differentiation of Human Induced Pluripotent Stem Cells into Cardiomyocyte
Human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are a novel s
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Chorioallantoic Membrane (CAM) Assay
8 eggs per day, day 7- day 13cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS
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转决定(transdetermination)
一般胚胎细胞一旦决定, 沿着特定类型进行分化的方向是稳定的, 但在果蝇中发现了某种突变体或培养的成虫盘细胞中有时会出现不按已决定的分化类型发育, 而生长出不是相
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Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
Reporter gene plasmids have been used extensively to monitor gene expression and
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B-Cell Development and Pre-B-1 Cell Plasticity In Vitro
B-lymphopoiesis in vivo is a very complex process that is influenced by cooperat
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Assessment and Quantification of Telomerase Enzyme Activity
The enzyme telomerase is activated in 80–90% of all human malignancies and immor
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Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay
Cell migration, essential in cancer progression, is a complex process comprising
