-
Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein int
-
Semipermeabilized Cells to Study Procollagen Assembly
This chapter will describe the preparation and use of a semipermeabilized (SP) c
-
Approaches and Species in the History of Vertebrate Embryology
Recent debates about model organisms echo far into the past; taking a longer vie
-
Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis
As the molecular mechanism of circadian clocks has reached high complexity, the
-
着丝粒(centromere)
染色体中连接两个染色单体, 并将染色单体分为两臂: 短臂(p)和长臂(q)的部位。由于此部位的染色质较细、内缢, 又叫主缢痕(primary constrict
-
不能不知的细胞培养的全攻略
相关专题基础篇-无菌操作基本技术1. 实验进行前，无菌室及无菌操作台(laminar flow) 以紫外灯照射30-60 分钟灭菌，以70 %ethanol 擦
-
Rapamycin-Induced Enhancement of Vaccine Efficacy in Mice
Th1 immunity protects against tuberculosis infection in mice and humans. The wid
-
Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells
Embryonic stem cells (ESCs) are pluripotent cells derived from preimplantation e
-
Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion
Ouabain, a toxic of vegetal origin used for centuries to treat heart failure, ha
-
Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila
Kinetochores are essential for the proper positioning, movement and segregation
-
Analysis of Common Mitochondrial DNA Mutations by Allele-Specific Oligonucleotide and Southern Blot
Mitochondrial disorders are clinically and genetically heterogeneous. There are
-
Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells
Research of stem cells will enable us to understand the development and function
-
应力纤维(stree fibers)
应力纤维又叫张力纤维，是真核细胞中广泛存在的一种较为稳定的束状纤维结构, 与骨骼肌中肌原纤维非常相像。应力纤维由大量平行排列的肌动蛋白组成,此外还含有其他在肌细
-
Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line
Cell culture is an invaluable tool for investigators in numerous fields. It faci
-
Conventional Micropipette-Based Patch Clamp Techniques
The patch clamp technique revolutionized the study of ion channels and is consid
