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Scale-Up of Suspension and Anchorage-Dependent Animal Cells
In this chapter, scale-up is described in a laboratory context (10–20 L), but th
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On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis
This chapter reviews the theory and methodological developments of on-line conce
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Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens
The process of bringing a new pharmacologically active drug to market is laborio
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Measurement of Geminin Activity in Xenopus Egg Extracts
Geminin is an unstable protein that inhibits DNA replication by interfering with
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Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequore
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RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium
Knockouts by homologous recombination are frequently used to investigate the fun
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合子基因（zygotic gene）
受精卵在胚胎发育过程中表达的基因称为合子基因，受母体基因产物的激活。合子基因的表达是胚胎模式形成的开端。合子基因分为若干组，表达顺序有先有后，它们之间有着严密的
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Phylogenetic Inference and Parsimony Analysis
Application of phylogenetic inference methods to comparative endocrinology studi
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Knock-Down of Gene Expression in Hematopoietic Cells
RNA interference (RNAi) is an evolutionarily conserved sequence-specific post-tr
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Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protei
The insulin-like growth factor-binding proteins (IGFBPs) are carrier proteins th
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Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products
Cell-cell electrofusion (CCE) is a process that involves forcing cells into clos
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A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS :1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2
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Long-Term Cryopreservation of Thylakoid Membranes
Chloroplast thylakoids are the site of photosynthetic light reactions, electron
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Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells
Since the mid-1980s, murine retroviral vectors have been used extensively by a n
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The Teratoma Assay: An In Vivo Assessment of Pluripotency
A teratoma is a nonmalignant tumor comprised of a disorganized mixture of cells
