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原位杂交的难题
问:各位好!我现在在大鼠脑组织的mRNA的原位杂交,测BDNF,DAB显色的时候有淡黄色,但是复染之后就是满视野蓝核了,只有零星的阳性结果,并且据文献报道以神经
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电穿孔
【求助】哪位大哥给指点一下电穿孔阿,xiexie参与者:vensentgwx哪位大哥给指点一下电穿孔阿,xiexie 告诉我哪里有介绍也行,我自己在网上没找到。
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Eradication of c-erbB-2-Positive Ovarian Cancer Cells Mediated by Intracellular Expression of Anti-c
Overexpression of erbB- 2 is important in the pathogenesis of a variety of human
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昆虫杆状病毒表达系统的研究进展
相关专题体外基因表达体外基因表达系统包括原核细胞系统和真核细胞系统。原核细胞系统主要是大肠杆菌细胞,它操作简便、周期短收益大及表达产物稳定,但是表达基因的相对分
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Single Nucleotide Polymorphism Microarray Analysis of Genetic Alterations in Cancer
The identification of structural genetic alterations, including DNA amplificatio
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无动物成分 植物源细胞因子
细胞治疗是将人体细胞经体外培养、诱导增殖活化后回输入人体的一种治疗肿瘤等疾病的方法,因安全有效,并能提高生活质量而广为人们所关注和采用。细胞治疗离不开细胞培养,
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报告基因检测技术
相关专题报告基因报告基因的种类很多,根据对其表达产物的分析检测方法的不同,可将其分为体外报告基因和体内报告基因。前者的分析需要在含有报告分子的细胞裂解液或细胞、
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成人干细胞和再生(第三部分)
“生命潜能:干细胞、克隆和再生”之Adult Stem Cells and Regeneration主持人:Peter J. Bruns, Ph.D.Vice
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CHO细胞表达系统
相关专题CHO细胞表达体系及其特点诞生于70年代末的基因工程药物因其具有其他药物无法比拟的优点,已迅速成为制药工业中一个引人瞩目的领域。1995年美国基因工程药
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Bisulfite Pyrosequencing
Bisulfite pyrosequencing is a sequencing-by-synthesis method used to quantitativ
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Tumor Suppression Through Angiogenesis Inhibition
In the early 1970s, Judah Folkman first proposed the hypothesis that tumor growt
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Flow Cytometric Monitoring of Fluorescent Drug Retention and Efflux
Laser flow cytometry has been used for monitoring cellular retention of fluoresc
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Expression and Function of P-Glycoprotein in Normal Tissues: Effect on Pharmacokinetics
ATP-binding cassette (ABC) drug efflux transporters limit intracellular concentr
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5-Fluorouracil Metabolizing Enzymes
The uracil analog 5-fluorouracil (5-FU) is used as part of combination therapy f
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Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
Conventionally, monoclonal antibodies (MAbs) are generated by fusing B cells fro