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Affinity chromatography (with HIS-tagged proteins)
Affinity chromatography (with HIS-tagged proteins)This protocol is described in
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WESTERN PROTOCOL
WESTERN PROTOCOL A. Preparation of cell lysatesCollect cells (confluent T-25) by
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蛋白酶抑制剂资料 Protease Inhibitors
Protease Inhibitors from Alberts' Lab Protocols (Kathy Miller/Chris Field)
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HISTONE KINASE ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western bl
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Isolation of Non-muscle Actin
General Preparation1. Prepare buffers and have them cold. Make 50X stock of Buff
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Western Blot/Tissue Preparation
OverviewThis is a protocol for preparing whole cell lysate from tissue, for west
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一步法甲醛胶连检测蛋白质相互作用Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with
INTRODUCTIONThis protocol describes a method for chemical cross-linking of prote
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Protein extraction from whole tissues for IEF
Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extract
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Western Blotting with Monoclonal Antibodies
Sample PreparationFor Protein Concentration Determination of Cell Culture Decant
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GST Fusion Protein Prep
Italics indicate optional steps especially useful for the analysis of untested p
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GSP融合蛋白的准备 GST Fusion Protein Prep
GST Fusion Protein PrepItalics indicate optional steps especially useful for the
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蛋白质和多肽MALDI-MS分析方法 General Method for MALDI-MS Analysis of Proteins and Peptides
ABSTRACTFor MALDI-TOF-MS analysis of proteins and peptides, samples are cocrysta
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Protein Expression and Purification Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These ce
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蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
Direct Enzymatic Digestion of Protein ComplexesSherry Niessen, Ian McLeod and Jo
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Home-made Taq Polymerase Purification
Home-made Taq Polymerase PurificationI also have the bacteria containing the clo
