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寡核苷酸的优化设计
关键词：寡核苷酸；优化设计 中图分类号：Q524 在核酸分子杂交、DNA序列测定和通过PCR放大DNA片段等实验中，都需要使用寡核苷酸作为探针或引物，而
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PCR from Tissue
collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube con
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Protocol for competitive RT-PCR
Protocol for competitive RT-PCRFor quantifying mRNA, we use a competitive RT-PCR
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Bisulfite-PCR for Restriction Analysis and/or Sequencing
Bisulfite-PCR for Restriction Analysis and/or SequencingProtocol written by Jean
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PCR常见问题分析与对策
1.PCR产物的电泳检测时间 一般为48h以内，有些最好于当日电泳检测，大于48h后带型不规则甚致消失。 2.假阴性，不出现扩增条带 PCR反应的关键环
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FAQs on Real-Time RT-PCR
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers ar
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Standard multiplex mixtures
Standard multiplex mixtures Over 75 primer pairs were chosen and a number of m
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THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR
TABLE OF CONTENTS 1. Abstract 2. Introductory statement 3. The key preparatory s
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RT-PCR Analysis
RT-PCR AnalysisSolutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1
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Making RNA probes for in situ hybridization
Make DNA templates via PCRDay 1It's preferable to start with constructs that
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PCR常见问题总汇(二）
克隆PCR产物 1)克隆PCR产物的最优条件是什么？ 最佳插入片段：载体比需实验确定。1：1（插入片段：载体）常为最佳比，摩尔数比1：8或8：1也行。应测定比值
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PURIFICATION OF PCR PRODUCTS WITH SEPHADEX
PURIFICATION OF PCR PRODUCTS WITH SEPHADEXPlace the sephadex measuring plate (Mu
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Protocol for competitive RT-PCR
For quantifying mRNA, we use a competitive RT-PCR protocol with internal standar
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Bisulfite Treatment of DNA
Bisulfite Treatment of DNAAdapted from Frommer et.al.*Dilute DNA (up to 2 mg) in
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定量RT-PCR （Quantitative RT-PCR）
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and a
