Detection of Carbonylated Proteins in 2-D SDS Page Separations
Protein carbonyls are an index of protein oxidation which, in turn, reflects the
Protein carbonyls are an index of protein oxidation which, in turn, reflects the interplay of oxidative stress and degradation of oxidatively modified proteins. Protein carbonyls are increased in brain proteins in aging and age-related neurodegenerative disorders, including Alzheimer's disease. In this chapter, we outline methods to detect protein carbonyls following two dimensional-based separation of brain proteins.
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Biochemical and Pharmacological Inhibition of mTOR by Rapamycin and an ATP-Competitive mTOR Inhibito
The mammalian target of rapamycin (mTOR) is the catalytic su...
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Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes
This chapter focuses on the analysis of RNA-protein complexe...
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