Single Enzyme Studies: A Historical Perspective
Single-molecule enzymology has a longer history than is often supposed, with the
Single-molecule enzymology has a longer history than is often supposed, with the first measurements being made as early as 1961. However, the development of new technologies has meant that most of the progress has been made in the last two decades. I review the development of single-molecule enzymology, focussing on five key papers which are milestones in the field. In particular, I discuss the 1961 paper by Boris Rotman, which made inventive use of what now seems like primitive technology, and continues to be influential to this day.
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DNA-Templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids: A Versatile Tool for
Single base-specific detection of DNA/RNA sequences is of im...
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Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Al
Single-strand conformational polymorphism analysis has been ...