Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 生物化学

生物化学

Gene Isolation by Exon Trapping

2025-03-29 生物化学 加入收藏
The technology of exon trapping, sometimes called exon amplification, strives to

The technology of exon trapping, sometimes called exon amplification, strives to exploit the phenomenon of mRNA splicing to discover genes directly from genomic DNA. There are three distinct exon trapping methodologies that differ simply in the genomic target of interest. The original experimental design was to capture isolated 3′-splice sites residing within fragments of genomic DNA (1 ), whereas later approaches focused on either complete internal exons (2 –5 ) or entire 3′-terminal exons (6 ). The requirement of complete, intact exons as targets has proven absolutely essential, and only the trapping of complete internal or 3′-terminal exons is practical.

文章底部广告位

文章评论

加载中~