In SituHybridization

In situ hybridization is a method used to localize nucleic acid sequences in cells or tissue sections. The first in situ hybridization experiments were described by two groups of researchers in 1969, John et al. and Gall and Pardue (reviewed in ref. 1 ). Radioactively labeled RNA probes were used to localize specific DNA sequences within the nuclei of Xenopus laevis (2 ).