FOUCHET METHOD stain for bile

1. Sections to distilled water.

2. Stain in Fouchets reagent 5 minutes.

3. Rinse in distilled water.

4. Counterstain with van gieson 5 minutes.

5. Rinse quickly in absolute alcohol.

6. Dehydrate, clear . Mount sections in DPX

Results

--Bile pigments Blue/Green

--Muscle Yellow

--Collagen Red

SOLUTIONS

Fouchet reagent

--25% aqueous trichloroacetic acid 25ml

--10% aqueous ferric chloride 25ml

Van Gieson Solution

--see "Elastic Van Gieson" Method.